The course took place on April 21st, 22nd, and 23rd, 2023 in Vale Dourado, located at km 50 of the Novo Airão road. Its main objective was to provide training for eco-tourism guides in the surrounding area of Vale Dourado, by providing theoretical and practical knowledge about the fauna, flora, and fungi of the Rio Negro Sustainable Development Reserve (RDS). The course included lectures, a workshop for children focusing on the biodiversity found in the RDS, and guided trails on frogs, reptiles, fungi, and ants.